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araA [2019-07-09 08:06:14]
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araA [2019-07-09 08:06:14]

L-arabinose isomerase
locus
BSU28800
pI
5.47
mw
56.37 kDa
protein length
496 aa Sequence Blast
gene length
1491 bp Sequence Blast
function
arabinose utilization
product
L-arabinose isomerase
essential
no
ec
5.3.1.4
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.6|Utilization of arabinan/ arabinose/ arabitol]

    • Gene

    Coordinates
    2,947,385 2,948,875

    The protein

    Catalyzed reaction/ biological activity

  • L-arabinose = L-ribulose (according to Swiss-Prot)

    • Protein family

  • arabinose isomerase family (according to Swiss-Prot)

    • Kinetic information

  • k (cat) of 14,504 min(-1) and k (cat)/K (m) of 121 min(-1) mM(-1) for L-arabinose [Pubmed|19727704]

    • Structure

  • [PDB|2AJT] (from ''Escherichia coli'', 51% identity, 71% similarity) [Pubmed|16756997]

    • Expression and Regulation

    Operons

    genes
    [gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]-[gene|E0525A4187D9C8A23F23BC8F883973896C8D295D|araB]-[gene|3859854178E573AE8ACCE6DF85E32C495D316DA1|araD]-[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]-[gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]-[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]-[gene|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[gene|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]-[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]
    description
    [Pubmed|9084180]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9084180], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12949161], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|A567466894AE9DE7CDE7816615433A37532297B5|AraR]: repression, [Pubmed|10417639], in [regulon|A567466894AE9DE7CDE7816615433A37532297B5|AraR regulon]

    • regulation

  • induced by arabinose ([protein|search|AraR]) [Pubmed|10417639]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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    Biological materials

    Mutant

  • BKE28800 ([gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT, downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT
  • BKK28800 ([gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT, downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT

    • References

  • 16585763,11418559,9084180,12949161,2500424,10417639,12949161,9084180,19727704